Difference between revisions of "Part:BBa M50039:Experience"
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| − | + | Escherichia coli (E. coli) naturally possesses a pathway to respond within a few minutes to sudden copper metal induction. 4 The main purpose of this system is to protect the cell from copper shock. The membrane protein cusS and cytoplasmic protein cusR are always present at low levels within the cell. The presence of copper phosphorylates the membrane protein cusS, which then trans-phosphorylates cytoplasmic cusR. Once cusR is activated, it binds to the appropriate sequence within the cusR-inducible promoter region, initiating bidirectional transcription of cusS and cusR. This pathway increases cusS and cusR concentration in the presence of copper, acting as a positive feedback loop that allows the cell to display an amplified response to toxic copper ions. These proteins then go on to activate downstream proteins that bind the copper ions to inactivate them, thereby increasing the cell’s survivability in copper ion solutions.<BR> | |
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Revision as of 21:08, 11 December 2016
Escherichia coli (E. coli) naturally possesses a pathway to respond within a few minutes to sudden copper metal induction. 4 The main purpose of this system is to protect the cell from copper shock. The membrane protein cusS and cytoplasmic protein cusR are always present at low levels within the cell. The presence of copper phosphorylates the membrane protein cusS, which then trans-phosphorylates cytoplasmic cusR. Once cusR is activated, it binds to the appropriate sequence within the cusR-inducible promoter region, initiating bidirectional transcription of cusS and cusR. This pathway increases cusS and cusR concentration in the presence of copper, acting as a positive feedback loop that allows the cell to display an amplified response to toxic copper ions. These proteins then go on to activate downstream proteins that bind the copper ions to inactivate them, thereby increasing the cell’s survivability in copper ion solutions.
Stanford Location
Plasmid Name: DNAse pColi P1
DNA 2.0 Gene #: 273824
Organism: E. coli
Device Type: Sensor
Glycerol Stock Barcode: 0133027170
Box Label: BIOE44 F16
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