Difference between revisions of "Part:BBa M50051"
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This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG. | This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
LsrR is a repressor protein that inhibits the expression of the Lsr operon. By preventing the expression of the Lsr operon, LsrR reduces the intake of Autoinducer-2, an important quorum sensing molecule that is conserved throughout a wide variety of bacteria species. | LsrR is a repressor protein that inhibits the expression of the Lsr operon. By preventing the expression of the Lsr operon, LsrR reduces the intake of Autoinducer-2, an important quorum sensing molecule that is conserved throughout a wide variety of bacteria species. |
Revision as of 17:14, 11 December 2016
IPTG Inducible Plasmid for the Expression of LsrR Repressor
This is a IPTG-inducible plasmid that encodes the expression of an LsrR repressor for the Lsr operon in E. coli. Although there are endogenous sources of LsrR, the plasmid allows for a controlled over-expression of protein through IPTG.
Usage and Biology
LsrR is a repressor protein that inhibits the expression of the Lsr operon. By preventing the expression of the Lsr operon, LsrR reduces the intake of Autoinducer-2, an important quorum sensing molecule that is conserved throughout a wide variety of bacteria species.
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 1134
- 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 1134
- 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 1134
- 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 1134
- 1000COMPATIBLE WITH RFC[1000]