Difference between revisions of "Part:BBa M50048"
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| − | + | This is a riboswitch consisting of an RNA aptamer that recognizes atrazine, and a ribosome binding site. It is based on BBa_J89000, with the theophylline aptamer exchanged for an atrazine aptamer. In the absence of atrazine, the RNA transcript of this part has excessive base pairing in the 5’ UTR, folding the RNA to block binding at the RBS and thus prevent translation. When atrazine is present, it binds to the aptamer sequence, altering the shape of the transcript to expose the RBS and allow translation of any downstream protein sequences. | |
Revision as of 02:51, 11 December 2016
This is a riboswitch consisting of an RNA aptamer that recognizes atrazine, and a ribosome binding site. It is based on BBa_J89000, with the theophylline aptamer exchanged for an atrazine aptamer. In the absence of atrazine, the RNA transcript of this part has excessive base pairing in the 5’ UTR, folding the RNA to block binding at the RBS and thus prevent translation. When atrazine is present, it binds to the aptamer sequence, altering the shape of the transcript to expose the RBS and allow translation of any downstream protein sequences.