Difference between revisions of "Part:BBa M50039:Design"

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===Design Notes===
 
===Design Notes===
none
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We designed a genetic construct where the first half consisted of the cusR inducible promoter, a strong ribosome binding site (RBS) listed in the Parts Registry (BBa_B0035) and thecusR gene sourced from NCBI.
 
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Revision as of 23:32, 11 December 2016


E. coli positive feedback cusR producer (short promoter)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 53
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 53
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 53
    Illegal AgeI site found at 243
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed a genetic construct where the first half consisted of the cusR inducible promoter, a strong ribosome binding site (RBS) listed in the Parts Registry (BBa_B0035) and thecusR gene sourced from NCBI.


Source

References