Difference between revisions of "Part:BBa K2012015"

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<partinfo>BBa_K2012015 parameters</partinfo>
 
<partinfo>BBa_K2012015 parameters</partinfo>
 
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<span id="IGEM 2021 XHD- Wuhan-B-China"><br><br><br><br><br></span>
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==iGEM 2021 XHD-Wuhan-B-China, new documentation (For Bronze)==
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<h3><b>Group: XHD-Wuhan-B-China</b></h3>
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<h3><b>Author: Kening Chen</b></h3>

Revision as of 04:35, 1 October 2021


PcpcG2-172, a modified PcpcG2 promoter

PcpcG2 promoter is a 238bp green-light activated promoter in Synechocystis PCC 6803(Part:BBa_K592003). The full length promoter is comprised of a G-box region, a CcaR-P activated promoter, and a constitutive promoter, which contributes to the leakiness under red light and low dynamic range. Therefore, we constructed PcpcG2-172, a 172bp truncated cpcG2 promoter deleted for the constitutive promoter.(For more detail, please view our wiki: "http://2016.igem.org/Team:HZAU-China/Experiments" )

 

Figure 1. Fluorescence assay of CcaS-CcaR system with PcpcG2-172 (BBa_K2012015) in CL1 (△EnvZ), and PCB (△CcaS) as chromophore.

Fluorescence under green light is 1.81-folds of red light, proving that green light activates output expression, the device works well. PcpcG2-172 shows high efficiency.




Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]

iGEM 2021 XHD-Wuhan-B-China, new documentation (For Bronze)

Group: XHD-Wuhan-B-China

Author: Kening Chen