Difference between revisions of "Part:BBa K2145001:Experience"

Revision as of 00:03, 31 October 2016

This experience page is provided so that any user may enter their experience using this part.

Applications of BBa_K2145001

Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.

User Reviews

UNIQ2e0029cf6b511932-partinfo-00000000-QINU UNIQ2e0029cf6b511932-partinfo-00000001-QINU

@@ Line 5: / Line 5: @@
 ===Applications of BBa_K2145001===
-Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
 [[File:Splates95102.png]]<br>
+Qualitatively, we noticed that none of the colonies containing this plasmid showed BOTH visible RFP and visible GFP -- each colony was either red, green, or neither, but never red and green (see GG96 above). Fluorescence was observed even without the addition of inducers (IPTG and ATc), even though the reporters should be under IPTG and ATc control. We attribute this to insufficient repression by LacI and TetR.
 [[File:Alverno_ca_96.png]]