Difference between revisions of "Part:BBa K2113002"
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Latest revision as of 22:58, 30 October 2016
temperature sensitive system with lambda pr promoter under cI regulation
It is a construct which contains a complete temperature sensitive system with lambda pr as a promoter (BBa_K608351) activated at 42 degree celcius with gfp (BBa_E0040) as its reporter to study the expression at various temperatures.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NotI site found at 781 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 1618
RESULTS -SVCE_Chennai 2016
temperature sensitive system with lambda pr promoter under cI regulation We constructed the λpR promoter with GFP in order to study the expression at 37degree Celsius , 42 degrees Celsius and 47 degree celsius. The fluorescence was measured and plotted as a graph. It was found that expression was higher at 42 degrees Celsius. And size conformation was done and the band was found at 3600bp.
