Difference between revisions of "Part:BBa K2145000"
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<partinfo>BBa_K2145000 short</partinfo> | <partinfo>BBa_K2145000 short</partinfo> | ||
− | + | This part contains two fluorescent protein expression units (for RFP and GFP) with promoters, RBSs, CDSs, and terminators, both facing forward, with a random 500bp spacer between the two reporters. Parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled with golden gate assembly to make the full part. This composite part is designed to demonstrate the effect, if any, of a 500 bp spacer between genes on supercoiling generated by the two genes, and therefore on interference between the genes. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> |
Revision as of 22:56, 30 October 2016
GFP/RFP reporter (Forward/Forward) with random 500bp spacer
This part contains two fluorescent protein expression units (for RFP and GFP) with promoters, RBSs, CDSs, and terminators, both facing forward, with a random 500bp spacer between the two reporters. Parts were amplified from iGEM Registry (BBa_J04450 (RFP) and BBa_I13522 (GFP)) and assembled with golden gate assembly to make the full part. This composite part is designed to demonstrate the effect, if any, of a 500 bp spacer between genes on supercoiling generated by the two genes, and therefore on interference between the genes.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 2226
Illegal AgeI site found at 2338 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 724