Difference between revisions of "Part:BBa K2139010"

Latest revision as of 21:04, 30 October 2016

Gluc1C with Terminator

Ba_K2139010 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C along with a double terminator. Gluc1C is a monomeric enzyme capable of catalyzing decomposition of cellobiose down to two glucose monomers. It can be used in conjunction with an exo-beta-1,4-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized by IDT for use in Caulobacter crescentus and is codon optimized.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 264
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1138

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 <partinfo>BBa_K2139010 short</partinfo>
-Ba_K2139010 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C along with a double terminator. Gluc1C is a monomeric enzyme capable of catalyzing decomposition of cellobiose down to two glucose monomers. It can be used in conjunction with an exo-beta-1,4-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
+Ba_K2139010 encompass the coding region for an 1,4-beta-glucosidase known as Gluc1C along with a double terminator. Gluc1C is a monomeric enzyme capable of catalyzing decomposition of cellobiose down to two glucose monomers. It can be used in conjunction with an exo-beta-1,4-glucanase and endo-beta-1,4-glucanase for the conversion of cellulose to glucose. The genbank ascension number for this protein is JQ713769.1 (DNA), AFQ36783.1 (amino acid), and a PDB code of 2O9R_A. This construct was synthesized by IDT for use in Caulobacter crescentus and is codon optimized.
 Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154