Difference between revisions of "Part:BBa K2139005"

Latest revision as of 20:56, 30 October 2016

Ptac RBS Endo5a

Endo5a Expression Cassette without Terminator BBa_K2139005 encompass the coding region for an endo-beta-1,4-glucanase known as Endo5A along with Ptac and an RBS. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exo-beta-1,4-glucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX (homolog). This construct was synthesized by IDT for use in Caulobacter crescentus and is codon optimized.

Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal XhoI site found at 1062
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal NgoMIV site found at 493
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI site found at 390

@@ Line 4: / Line 4: @@
 Endo5a Expression Cassette without Terminator
-BBa_K2139005 encompass the coding region for an endo-beta-1,4-glucanase known as Endo5A along with Ptac and an RBS. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exo-beta-1,4-glucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX (homolog). This construct was synthesized for use in Caulobacter crescentus and is codon optimized to contain a high GC content.
+BBa_K2139005 encompass the coding region for an endo-beta-1,4-glucanase known as Endo5A along with Ptac and an RBS. Endo5a catalyzes the conversion of cellulose into smaller fragments by making internal cuts in the cellulose chain. It can be used in conjunction with exo-beta-1,4-glucanases and beta-1,4-glucosidases for the direct conversion of cellulose into monomeric glucose. The protein is monomeric with an optimal temperature of 50 degrees Celsius and an optimal pH ranging from 6 to 8. The genbank ascension number for this protein is HQ657203.1 (DNA), AEB00655.1 (amino acid), and a PDB code of 1VRX (homolog). This construct was synthesized by IDT for use in Caulobacter crescentus and is codon optimized.
 Literature data for this part can be found http://www.sciencedirect.com/science/article/pii/S1046592812003154