Difference between revisions of "Part:BBa K2042006:Experience"
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| − | iGEM Evry 2016 cloned a GFP on the pSEVA2311 vector containing this promoter and transformed Pseudomonas putida to assess qualitative fluorescence. Quantitative characterization on levels of fluorescence in different concentrations and time | + | iGEM Evry 2016, with the collaboration of iGEM Paris Bettencourt 2016, cloned a GFP on the pSEVA2311 vector containing this promoter and transformed Pseudomonas putida to assess qualitative fluorescence. Quantitative characterization on levels of fluorescence in different concentrations and time could not be provided due to limitations of cyclohexanone manipulation. However, this promoter has been well documented by De Lorenzo lab. [1] |
Latest revision as of 00:47, 30 October 2016
iGEM Evry 2016, with the collaboration of iGEM Paris Bettencourt 2016, cloned a GFP on the pSEVA2311 vector containing this promoter and transformed Pseudomonas putida to assess qualitative fluorescence. Quantitative characterization on levels of fluorescence in different concentrations and time could not be provided due to limitations of cyclohexanone manipulation. However, this promoter has been well documented by De Lorenzo lab. [1]
References:
[1] Benedetti, Ilaria, Pablo I. Nikel, and Víctor de Lorenzo. "Data on the standardization of a cyclohexanone-responsive expression system for Gram-negative bacteria." Data in brief 6 (2016): 738-744.
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