Difference between revisions of "Part:BBa K2179003"

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<h5>Translational Unit for Chlorite Dismutase (Cld)</h5>
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<h5>Translational Unit for Chlorite Dismutase Cld(-SP)</h5>
 
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Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.  
 
Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.  

Revision as of 23:43, 29 October 2016

Translational Unit for Chlorite Dismutase Cld(-SP)

Chlorite Dismutase is an enzyme which catalyzes the reduction of chlorite ions to oxygen gas and chloride ions. Cld(-SP) is a translational unit which lacks the original Chlorite Dismutase gene's putative signal peptide, and also has a polyhistidine tag appended on it for the purpose of protein purification. The activity of this enzyme can be observed as bubbling of a liquid culture when transformed cells are combined with compounds containing the chlorite oxyanion.

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 807
    Illegal BsaI.rc site found at 6