Difference between revisions of "Part:BBa K1110003:Experience"
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===Applications of BBa_K1110003=== | ===Applications of BBa_K1110003=== | ||
| − | Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. | + | Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps. |
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[[File:T--Georgia_State--Pichia_Mambalgin1_Coomassie.png|none|600px]] | [[File:T--Georgia_State--Pichia_Mambalgin1_Coomassie.png|none|600px]] | ||
Revision as of 23:18, 29 October 2016
Applications of BBa_K1110003
Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. Additionally, further purification of the supernatant was not needed after initial centrifugation. Thus, protein expression of Mambalgin-1 in P. pastoris required a minimal amount of steps.
Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression. "Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes"
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