Difference between revisions of "Part:BBa K1110003:Experience"
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Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. | Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size. | ||
| + | [[File:T--Georgia_State--Pichia_Mambalgin1_Coomassie.png|none|600px]] | ||
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| − | [[File:T--Georgia_State-- | + | [[File:T--Georgia_State--Mambalgin_Ppastoris_Coomassie.png]] |
| + | '''Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.''' | ||
| + | "Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes" | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 23:03, 29 October 2016
Applications of BBa_K1110003
Mambalgin-1 was expressed in Pichia pastoris and confirmed with colony PCR. Expression was shown with a western blot and coomassie stain. The coomassie stain resulted in a band near 9kDa, which was the expected band size.
Figure 1: Coomassie Gel Stain of Mambalgin-1 in Pichia pastoris Protein Expression.
"Coomassie stain of SDS-PAGE run at 100 volts for 30 minutes"
User Reviews
UNIQd82975db60c313e3-partinfo-00000000-QINU UNIQd82975db60c313e3-partinfo-00000001-QINU