Difference between revisions of "Part:BBa K1952010"
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Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion). | Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion). | ||
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| + | Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells. | ||
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Revision as of 15:48, 29 October 2016
Hydrazine Synthase subunit gamma (Kust-2859) with LacZ reporter
Hydrazine Synthase subunit gamma (Kust-2859) with strong RBS under lactose-inducible promoter, followed by LacZ-alpha reporter gene (protein fusion).
Preliminary test indicates that reporter gene is expressed, indicating that the fusion protein is expressed, active, and non-toxic to the cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 445
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 407
Illegal XhoI site found at 1474 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 568
Illegal AgeI site found at 1461 - 1000COMPATIBLE WITH RFC[1000]