Difference between revisions of "Part:BBa I739001"
Stefan Luzi (Talk | contribs) |
Stefan Luzi (Talk | contribs) |
||
| Line 12: | Line 12: | ||
===Testing=== | ===Testing=== | ||
| − | Checked for uniqueness of restriction enzyme cleavage sites: | + | <p>Checked for uniqueness of restriction enzyme cleavage sites: |
| − | + | Eco: ok<br> | |
| − | Eco: ok | + | Xba: ok<br> |
| − | Xba: ok | + | Spe: ok<br> |
| − | Spe: ok | + | Pst: ok<br> |
| − | Pst: ok | + | </p><br> |
| − | + | ||
<!-- Add more about the biology of this part here | <!-- Add more about the biology of this part here | ||
===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 11:50, 11 October 2007
Constitutive expression cassette for TetR +LVA (J23100.B0034.C0040.B0015)
Part Structure
The Biobrick encodes LVA-tagged tetR (BBa_C0040) under control of the constitutive promoter BBa_J23100 followed by the ribosome binding site BBa_B0034. The transcription of tetR is terminated by the double terminator BBa_B0015.
Mode of Action
TetR binds to the pTet regulator (BBa_R0040) and represses its action. If the inducer [http://openwetware.org/wiki/ATc anhydrotetracycline (ATc)] is added, tetR action is inhibited and the promoter gets derepressed.
Purpose
This Biobrick was designed for the [http://parts.mit.edu/igem07/index.php/ETHZ ETHZ iGEM 2007 project] and belongs to the constitutive part of the system. In the project describtion, this part is also termed Part 1. The constitutively synthesized TetR interacts with .... A similar (but not used) construct in this context is BBa_Q04400.
Testing
Checked for uniqueness of restriction enzyme cleavage sites:
Eco: ok
Xba: ok
Spe: ok
Pst: ok
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]