Difference between revisions of "Part:BBa K1800000"
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| − | Mambalgin is a potent analgesic protein found in the venom of the <i>Dendroaspis polylepis</i> (Black Mamba snake). Mambalgin-1, the version found here, is a 3-finger toxin consisting of 57 amino acid residues forming 3 loops around a core in the shape of a hand. This protein has been found to inhibit acid-sensing ion channels (ASICs) in the central and peripheral nervous systems of mice through intraplanter and intrathecal injections. The inhibition of ASICs – important contributors to the pain pathway in both mice and humans – decreases the sensitivity of nociceptive neurons to the perception of pain. The potency of mambalgin has been compared to the drug morphine. Unlike morphine, however, mambalgin has not shown an increase in tolerance over time (Diochot et al., 2012). Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes. We designed BBa_K1800000 part for expression in E.<i>coli</i> and Pichia <i> | + | Mambalgin is a potent analgesic protein found in the venom of the <i>Dendroaspis polylepis</i> (Black Mamba snake). Mambalgin-1, the version found here, is a 3-finger toxin consisting of 57 amino acid residues forming 3 loops around a core in the shape of a hand. This protein has been found to inhibit acid-sensing ion channels (ASICs) in the central and peripheral nervous systems of mice through intraplanter and intrathecal injections. The inhibition of ASICs – important contributors to the pain pathway in both mice and humans – decreases the sensitivity of nociceptive neurons to the perception of pain. The potency of mambalgin has been compared to the drug morphine. Unlike morphine, however, mambalgin has not shown an increase in tolerance over time (Diochot et al., 2012). Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes. We designed BBa_K1800000 part for expression in E.<i>coli</i> and Pichia <i>pastorsis</i> . |
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Revision as of 07:03, 29 October 2016
Mambalgin-1 for E. coli
Brief introduction of Mambalgin Protein
Mambalgin is a potent analgesic protein found in the venom of the Dendroaspis polylepis (Black Mamba snake). Mambalgin-1, the version found here, is a 3-finger toxin consisting of 57 amino acid residues forming 3 loops around a core in the shape of a hand. This protein has been found to inhibit acid-sensing ion channels (ASICs) in the central and peripheral nervous systems of mice through intraplanter and intrathecal injections. The inhibition of ASICs – important contributors to the pain pathway in both mice and humans – decreases the sensitivity of nociceptive neurons to the perception of pain. The potency of mambalgin has been compared to the drug morphine. Unlike morphine, however, mambalgin has not shown an increase in tolerance over time (Diochot et al., 2012). Furthermore, recombinant purification of Mambalgin could assist in developing anti-venom without the attendant risk of harvesting venom directly from snakes. We designed BBa_K1800000 part for expression in E.coli and Pichia pastorsis .