Difference between revisions of "Part:BBa K1915001:Experience"

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After successful sequencing results in DH5&alpha,
  
  

Revision as of 02:02, 29 October 2016


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Applications of BBa_K1915001

After successful sequencing results in DH5&alpha,


T--Georgia State--LT10 Coomassie3.png

Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction. Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.



T--Georgia State--LTNF10 Western.png

Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction. Western blot of SDS-PAGE with His antibody. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.

Elution 2 exhibited the strongest signal after visualization of western blot.

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