Difference between revisions of "Part:BBa K1915001:Experience"
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[[File:T--Georgia State--LTNF10 Western.png|frame|Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction | [[File:T--Georgia State--LTNF10 Western.png|frame|Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction | ||
+ | Ni-NTA column.]] | ||
Western blot of SDS-PAGE with His antibody | Western blot of SDS-PAGE with His antibody | ||
− | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from | + | Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from |
− | + | ||
Elution 2 exhibited the strongest signal | Elution 2 exhibited the strongest signal |
Revision as of 01:14, 29 October 2016
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Applications of BBa_K1915001
Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
Western blot of SDS-PAGE with His antibody Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from
Elution 2 exhibited the strongest signal
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UNIQ01b43f6bd366b6ab-partinfo-00000000-QINU UNIQ01b43f6bd366b6ab-partinfo-00000001-QINU