Difference between revisions of "Part:BBa K1915001:Experience"

(Applications of BBa_K1915001)
(Applications of BBa_K1915001)
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[[File:T--Georgia_State--LT10_Coomassie3.png]]
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[[File:T--Georgia_State--LT10_Coomassie3.png|frame|Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction
Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction
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Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes.  
 
Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes.  
Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.
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Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.]]
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Revision as of 00:59, 29 October 2016


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Applications of BBa_K1915001

Figure 1: Coomassie Gel Stain of LTNF10 in BL21 E. coli after Protein Induction Coomassie gel stain of SDS-PAGE run at 100 volts for 45 minutes. Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.


T--Georgia State--LTNF10 Western.png Figure 2: Western blot of LT10 in BL21 E. coli after Protein Induction Western blot of SDS-PAGE with His antibody Fraction P3 consisted of sonicated lysate. Fractions F1, Wash 1, Wash 2, Elution 1, 2, 3, and 4 from Ni-NTA column.

Elution 2 exhibited the strongest signal

User Reviews

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