Difference between revisions of "Part:BBa M50021:Design"

 
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
  
−
 
+
All parts are optimized for expression in E. coli.  Terminator was added to prevent expression of the GFP gene included in the plasmid this construct was going to be inserted into
  
  
  
 
===Source===
 
===Source===
−
 
+
RBS and terminator are derived from bacteriophage T7; sequences provided by GeneDesigner software
−
 
+
Promoter comes from groE gene in E. coli
−
 
+
d-limonene synthase gene was sequenced from Citrus unshiu, then optimized for E. coli
  
 
===References===
 
===References===
 +
GeneDesigner
 +
Wang & deHaseth, 2003 (promoter)
 +
Lindler, 1994 (promoter)
 +
Shimada, 2005 (d-limonene synthase)

Revision as of 19:16, 11 December 2016


Heat-inducible d-Limonene synthase


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1895
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1600
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1597
    Illegal SapI.rc site found at 319
    Illegal SapI.rc site found at 1572


Design Notes

All parts are optimized for expression in E. coli. Terminator was added to prevent expression of the GFP gene included in the plasmid this construct was going to be inserted into


Source

RBS and terminator are derived from bacteriophage T7; sequences provided by GeneDesigner software Promoter comes from groE gene in E. coli d-limonene synthase gene was sequenced from Citrus unshiu, then optimized for E. coli

References

GeneDesigner Wang & deHaseth, 2003 (promoter) Lindler, 1994 (promoter) Shimada, 2005 (d-limonene synthase)