Difference between revisions of "Part:BBa K2123108"

Revision as of 20:42, 27 October 2016

Improved Mer Operon Device with novel regulated promoter

Overview

In 2014, our team constructed a synthetic genetic circuit which reached 74% of mercury bioremediation. Now, this composite part was developed to improve the mer operon expression, related to mercury metabolism, aiming to increase Hg. In order to do so, we designed 8 new promoters regulated by mer operon system. This composite part is the final device with the best of the regulated promoters. We achieved 97% of mercury bioremediation and validated under real world conditions, on the first bioreactor to Hg bioremediation. You can see the promoter characterization here: http://2016.igem.org/Team:UFAM-UEA_Brazil/Project/NewPromoters

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 24
Illegal NheI site found at 28
Illegal NheI site found at 32
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 20:41, 27 October 2016 (view source) Mctastolfi (Talk \| contribs) ← Older edit		Revision as of 20:42, 27 October 2016 (view source) Mctastolfi (Talk \| contribs) Newer edit →
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	==Overview==		==Overview==
−	In 2014, our team constructed a synthetic genetic circuit which reached 74% of mercury bioremediation. Now, this composite part was developed to improve the mer operon expression, related to mercury metabolism, aiming to increase Hg. In order to do so, we designed 8 new promoters regulated by mer operon system. This composite part is the final device with the best of the regulated promoters You can see the promoter characterization here: http://2016.igem.org/Team:UFAM-UEA_Brazil/Project/NewPromoters	+	In 2014, our team constructed a synthetic genetic circuit which reached 74% of mercury bioremediation. Now, this composite part was developed to improve the mer operon expression, related to mercury metabolism, aiming to increase Hg. In order to do so, we designed 8 new promoters regulated by mer operon system. This composite part is the final device with the best of the regulated promoters. We achieved 97% of mercury bioremediation and validated under real world conditions, on the first bioreactor to Hg bioremediation. You can see the promoter characterization here: http://2016.igem.org/Team:UFAM-UEA_Brazil/Project/NewPromoters