Difference between revisions of "Part:BBa K2027013:Design"
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===Design Notes=== | ===Design Notes=== | ||
Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant | Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant | ||
| − | [[File: | + | [[File:AroG_Mut1_SV|500px|thumb|left|alt text]] |
| − | + | ||
===Source=== | ===Source=== | ||
Revision as of 15:03, 27 October 2016
Mutant Feedback-Resistant Version of AroG (Pro150Leu)
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 934
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 241
Design Notes
Used site-directed mutagenesis to confirm mutation Pro150Leu which makes DAHP Synthase feedback-resistant
File:AroG Mut1 SV
alt text
Source
https://parts.igem.org/Part:BBa_K1060000