Difference between revisions of "Part:BBa K1976053"
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<b>Figure 2</b>: SDS-PAGE of the Colicin E2 DNase domain, the blue arrow marks the band of the expression. From left to right: Top10 E. coli, Top10 E. coli transformed with BBa_K1976052 and Top10 E. coli transformed with BBa_K1976053. | <b>Figure 2</b>: SDS-PAGE of the Colicin E2 DNase domain, the blue arrow marks the band of the expression. From left to right: Top10 E. coli, Top10 E. coli transformed with BBa_K1976052 and Top10 E. coli transformed with BBa_K1976053. | ||
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Revision as of 19:50, 26 October 2016
Colicin E2 DNase trypsin fragment and immunity protein Im2 generator with anderson promoters
Generator of colicin E2 DNase trypsin fragment BBa_K1976049 and immunity protein Im2 BBa_K1976026.
Figure 1: The structure of the Im2 immunity protein and Colicin E2 in a complex.
Usage
This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to the DNase fragment.
Characteristics
Figure 2: SDS-PAGE of the Colicin E2 DNase domain, the blue arrow marks the band of the expression. From left to right: Top10 E. coli, Top10 E. coli transformed with BBa_K1976052 and Top10 E. coli transformed with BBa_K1976053.
| Molecular Weight |
Colicin E2 Dnase domain
15.2 kDa Immunity Protein 10.0 kDa |
| Residues | Colicin E2 Dnase domain 133 |
| pI |
Colicin E2 Dnase domain
9.06 Immunity Protein 4.66 |
| [Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php] |
Characteristics
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30
Illegal NheI site found at 492
Illegal NheI site found at 515 - 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 696
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 107
- 1000COMPATIBLE WITH RFC[1000]
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007