Difference between revisions of "Part:BBa K2116004"
| Line 1: | Line 1: | ||
| − | + | __NOTOC__ | |
| − | __NOTOC__ | + | |
<partinfo>BBa_K2116004 short</partinfo> | <partinfo>BBa_K2116004 short</partinfo> | ||
<p> | <p> | ||
| − | We constructed a selection of AND gates responding to nitric oxide (NO) and 3OC6HSL (AHL). They were designed using the previously described NorV promoter [[Part:BBa_K1153000]]. | + | We constructed a selection of AND gates responding to nitric oxide (NO) and 3OC6HSL (AHL). They were designed using the previously |
| − | This Promoter (from here on refered to as PnorV) is the native promoter controlling the nitric oxide reduction operon (norRVW) in <i>E. coli</i> [1]. Its transcriptional regulator, | + | described NorV promoter [[Part:BBa_K1153000]]. This Promoter (from here on refered to as PnorV) is the native promoter controlling |
| − | NorR, can bind to nitric oxide and activate gene expression. | + | the nitric oxide reduction operon (norRVW) in <i>E. coli</i> [1]. Its transcriptional regulator, NorR, can bind to nitric |
| − | Using the distinct properties of esaboxes, PnorV was designed to also be responsive to AHL, giving it an AND gate behaviour. | + | oxide and activate gene expression. Using the distinct properties of esaboxes, PnorV was designed to also be responsive |
| − | An esabox is an 18bp sequence to which the transcriptional regulator EsaR [[Part:BBa_K2116001]] can bind. Transcription can be initiated by the specific AHL EsaR responds | + | to AHL, giving it an AND gate behaviour. An esabox is an 18bp sequence to which the transcriptional regulator EsaR [[Part:BBa_K2116001]] |
| − | to [N-(3-oxo-hexanoyl)-L-homoserine lactone]. By placing one, two or three esaboxes at different positions in the vicinity of PnorV, different specificities for AHL and | + | can bind. Transcription can be initiated by the specific AHL EsaR responds to [N-(3-oxo-hexanoyl)-L-homoserine lactone]. |
| − | were reached. We created and characterized a collection of these kind of AND gates: <br> | + | By placing one, two or three esaboxes at different positions in the vicinity of PnorV, different specificities for AHL and |
| + | NO were reached. We created and characterized a collection of these kind of AND gates: <br> | ||
| − | <ul> | + | <ul> |
| − | <li> [[Part:BBa_K2116004]] | + | <li> [[Part:BBa_K2116004]] |
| − | <li> [[Part:BBa_K2116005]] | + | <li> [[Part:BBa_K2116005]] |
| − | <li> [[Part:BBa_K2116006]] | + | <li> [[Part:BBa_K2116006]] |
| − | <li> [[Part:BBa_K2116012]] | + | <li> [[Part:BBa_K2116012]] |
| − | <li> [[Part:BBa_K2116013]] | + | <li> [[Part:BBa_K2116013]] |
| − | <li> [[Part:BBa_K2116014]] | + | <li> [[Part:BBa_K2116014]] |
| − | <li> [[Part:BBa_K2116007]] | + | <li> [[Part:BBa_K2116007]] |
| − | <li> [[Part:BBa_K2116008]] | + | <li> [[Part:BBa_K2116008]] |
| − | <li> [[Part:BBa_K2116068]] | + | <li> [[Part:BBa_K2116068]] |
| − | <li> [[Part:BBa_K2116015]] | + | <li> [[Part:BBa_K2116015]] |
| − | </ul> | + | </ul> |
</p> | </p> | ||
| Line 29: | Line 29: | ||
<h2> Characterization of this part </h2> | <h2> Characterization of this part </h2> | ||
| − | Data will be here before the final presentation at the jamboree. Apologies for the delay. | + | This AND gate has one esabox placed as a roadblock after PnorV. [[File:T--ETH Zurich--p21new.png]] |
| + | Data will be here before the final presentation at the jamboree. Apologies for the delay. | ||
<h2>References:</h2> | <h2>References:</h2> | ||
| Line 35: | Line 36: | ||
<ul> | <ul> | ||
| − | <li> [1] Gardner, A. M. "Regulation Of The Nitric Oxide Reduction Operon (Norrvw) In Escherichia Coli. ROLE OF Norr AND Sigma 54 IN THE NITRIC OXIDE STRESS RESPONSE". Journal of Biological Chemistry 278.12 (2003): 10081-10086.</i> | + | <li> [1] Gardner, A. M. "Regulation Of The Nitric Oxide Reduction Operon (Norrvw) In Escherichia Coli. ROLE OF Norr AND Sigma |
| − | <li> [2] Shong, Jasmine and Cynthia H. Collins. "Engineering The Esar Promoter For Tunable Quorum Sensing-Dependent Gene Expression". ACS Synth. Biol. 2.10 (2013): 568-575. </li> | + | 54 IN THE NITRIC OXIDE STRESS RESPONSE". Journal of Biological Chemistry 278.12 (2003): 10081-10086.</i> |
| + | <li> [2] Shong, Jasmine and Cynthia H. Collins. "Engineering The Esar Promoter For Tunable Quorum Sensing-Dependent Gene Expression". | ||
| + | ACS Synth. Biol. 2.10 (2013): 568-575. </li> | ||
| − | <!-- Add more about the biology of this part here | + | <!-- Add more about the biology of this part here |
===Usage and Biology=== | ===Usage and Biology=== | ||
<!-- --> | <!-- --> | ||
| − | <span class='h3bb'>Sequence and Features</span> | + | <span class='h3bb'>Sequence and Features</span> |
| − | <partinfo>BBa_K2116004 SequenceAndFeatures</partinfo> | + | <partinfo>BBa_K2116004 SequenceAndFeatures</partinfo> |
| − | <!-- Uncomment this to enable Functional Parameter display | + | <!-- Uncomment this to enable Functional Parameter display |
===Functional Parameters=== | ===Functional Parameters=== | ||
<partinfo>BBa_K2116004 parameters</partinfo> | <partinfo>BBa_K2116004 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
Revision as of 21:01, 26 October 2016
AND gate regulated by norR and esaR (one esabox)
We constructed a selection of AND gates responding to nitric oxide (NO) and 3OC6HSL (AHL). They were designed using the previously
described NorV promoter Part:BBa_K1153000. This Promoter (from here on refered to as PnorV) is the native promoter controlling
the nitric oxide reduction operon (norRVW) in E. coli [1]. Its transcriptional regulator, NorR, can bind to nitric
oxide and activate gene expression. Using the distinct properties of esaboxes, PnorV was designed to also be responsive
to AHL, giving it an AND gate behaviour. An esabox is an 18bp sequence to which the transcriptional regulator EsaR Part:BBa_K2116001
can bind. Transcription can be initiated by the specific AHL EsaR responds to [N-(3-oxo-hexanoyl)-L-homoserine lactone].
By placing one, two or three esaboxes at different positions in the vicinity of PnorV, different specificities for AHL and
NO were reached. We created and characterized a collection of these kind of AND gates:
- Part:BBa_K2116004
- Part:BBa_K2116005
- Part:BBa_K2116006
- Part:BBa_K2116012
- Part:BBa_K2116013
- Part:BBa_K2116014
- Part:BBa_K2116007
- Part:BBa_K2116008
- Part:BBa_K2116068
- Part:BBa_K2116015
Characterization of this part
This AND gate has one esabox placed as a roadblock after PnorV. 
Data will be here before the final presentation at the jamboree. Apologies for the delay.
References:
- [1] Gardner, A. M. "Regulation Of The Nitric Oxide Reduction Operon (Norrvw) In Escherichia Coli. ROLE OF Norr AND Sigma 54 IN THE NITRIC OXIDE STRESS RESPONSE". Journal of Biological Chemistry 278.12 (2003): 10081-10086.</i>
- [2] Shong, Jasmine and Cynthia H. Collins. "Engineering The Esar Promoter For Tunable Quorum Sensing-Dependent Gene Expression". ACS Synth. Biol. 2.10 (2013): 568-575.
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Sequence and Features
Assembly Compatibility: