Difference between revisions of "Part:BBa K1878004:Experience"
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<I>Rice iGEM 2016</I> | <I>Rice iGEM 2016</I> | ||
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| − | For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate | + | For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions. |
| − | [[Image:Fdhf_assay_670.jpg|thumb|left|800px|Fig 1. Results of part assay.]] | + | [[Image:Fdhf_assay_670.jpg|thumb|left|800px|Fig 1. Results of part assay in aerobic, and anaerobic, conditions.]] |
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Latest revision as of 07:33, 26 October 2016
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For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, the cofactor fdhA binds to formate, a metabolite in anaerobic respiration, to upregulate transcription downstream of fdhF. However, despite this description from literature, our assay results indicate that transcription was downregulated in anaerobic conditions. |
