Difference between revisions of "Part:BBa K1878005:Experience"
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===User Reviews===
 
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<I>Rice iGEM 2016</I>
 
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in <i>E.coli</i> under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.
 
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[[Image:FdhF_iRFP713_Hypoxia_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay.]]
 
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<partinfo>BBa_K1878005 AddReview number</partinfo>
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<I>Rice iGEM 2016</I>
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|width='60%' valign='top'|
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in <i>E.coli</i> under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.
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[[Image:FdhF_iRFP713_Hypoxia_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay.]]
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Revision as of 07:00, 26 October 2016


This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K1878005

User Reviews

UNIQe8a07c66bf347c25-partinfo-00000000-QINU UNIQe8a07c66bf347c25-partinfo-00000001-QINU

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.

Fig 1. Results of part assay.