Difference between revisions of "Part:BBa K1878004:Experience"
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For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> between fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF. | For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> between fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF. | ||
| − | [[Image:FdhF_iRFP670_Hypoxia_Assay. | + | [[Image:FdhF_iRFP670_Hypoxia_Assay.jpg|thumb|left|800px|Fig 1. Results of part assay.]] |
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 06:54, 26 October 2016
This experience page is provided so that any user may enter their experience using this part.
Please enter
how you used this part and how it worked out.
Applications of BBa_K1878004
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No review score entered. Rice iGEM 2016 |
For the 2016 Team Rice project, we built a biosensor construct in E. coli between fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF. File:FdhF iRFP670 Hypoxia Assay.jpg Fig 1. Results of part assay. |
User Reviews
UNIQc15533fc29ecb24a-partinfo-00000001-QINU UNIQc15533fc29ecb24a-partinfo-00000002-QINU