Difference between revisions of "Part:BBa K1878005:Experience"

Revision as of 06:58, 26 October 2016

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Applications of BBa_K1878005

User Reviews

No review score entered. Rice iGEM 2016

For the 2016 Team Rice project, we built a biosensor construct in E. coli with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.

Fig 1. Results of part assay.

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 <I>Rice iGEM 2016</I>
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-For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> between fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.
+For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> with fdhF and iRFP713 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in <i>E.coli</i> under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF.
 [[Image:FdhF_iRFP713_Hypoxia_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay.]]