Difference between revisions of "Part:BBa K1878004:Experience"
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<I>Rice iGEM 2016</I> | <I>Rice iGEM 2016</I> | ||
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| − | + | For the 2016 Team Rice project, we built a biosensor construct in <i>E. coli</i> between fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF. | |
| + | [[Image:FdhF_iRFP670_Hypoxia_Assay.jpeg|thumb|left|800px|Fig 1. Results of part assay.]] | ||
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===User Reviews=== | ===User Reviews=== | ||
Revision as of 06:54, 26 October 2016
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No review score entered. Rice iGEM 2016 |
For the 2016 Team Rice project, we built a biosensor construct in E. coli between fdhF and iRFP670 to fluoresce in the absence of oxygen. Specifically, formate is a metabolite that is generated in E.coli under anaerobic conditions, which binds to fdhA to induce transcription downstream of fdhF. File:FdhF iRFP670 Hypoxia Assay.jpeg Fig 1. Results of part assay. |
User Reviews
UNIQ14c09c3a99136181-partinfo-00000001-QINU UNIQ14c09c3a99136181-partinfo-00000002-QINU