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Revision as of 15:10, 25 October 2016

Colicin E2 DNase with B0034 and T7 promoter

Generator for the Colicin DNase domain BBa_K1976048.

Figure 1:Figure 5: growth of BL21 E. coli transformed with a): BBa_K1976054 and b): BBa_K1976055.


Usage

This part exhibited signs of leakage, that can kill the host if cells are transformed with it, that have a T7 promoter system, due to the endonuclease activity of the Colicin E2 DNase domain.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 66
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007