Difference between revisions of "Part:BBa K1976052"
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        <h1>Colicin E2 DNase domain and immunity protein Im2 generator with anderson promoters</h1>
  
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Generator of colicin E2 DNase domain <a href="https://parts.igem.org/Part:BBa_K197604">BBa_K1976048</a> and immunity protein Im2 <a href="https://parts.igem.org/Part:BBa_K1976026">BBa_K1976026</a>.
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<partinfo>BBa_K1976052 short</partinfo>
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<img style="width: 40%; height: 40%; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/parts/8/8b/Immuno_colicine2.png">
  
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Colicin E2 DNase domain and immunity Protein with Anderson promoters
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<b>Figure 1:</b> The structure of the Im2 immunity protein and Colicin E2 in a complex.
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===<h2>Usage</h2>===
  
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===Usage and Biology===
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This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to the DNase fragment.
  
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<span class='h3bb'>Sequence and Features</span>
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===<h2>Characteristics</h2>===
  
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===Functional Parameters===
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<partinfo>BBa_K1976052 parameters</partinfo>
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                    Molecular Weight
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                    Colicin E2 Dnase domain
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                    <br>15.2 kDa<br>
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                    Immunity Protein
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                    <br>10.0 kDa<br>
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                    Residues
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                    Colicin E2 Dnase domain
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                    133
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                    pI
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                    Colicin E2 Dnase domain
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                    <br>9.06<br>
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                    Immunity Protein
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                    <br>4.66<br>
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                    [Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php]
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===<h2>Characteristics</h2>===
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<h2>Sequence and Features</h2>
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<partinfo>BBA_K1976052 SequenceAndFeatures</partinfo>
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[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br>
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Revision as of 00:26, 26 October 2016

Colicin E2 DNase domain and immunity protein Im2 generator with anderson promoters

Generator of colicin E2 DNase domain BBa_K1976048 and immunity protein Im2 BBa_K1976026.

Figure 1: The structure of the Im2 immunity protein and Colicin E2 in a complex.


Usage

This part can be used to produce colicin without killing the host cell, as the immunity protein is produced more than equimolar to the DNase fragment.

Characteristics


Molecular Weight Colicin E2 Dnase domain
15.2 kDa
Immunity Protein
10.0 kDa
Residues Colicin E2 Dnase domain 133
pI Colicin E2 Dnase domain
9.06
Immunity Protein
4.66
[Data taken created in Snapgene and http://isoelectric.ovh.org/calculate.php]

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 477
    Illegal NheI site found at 500
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 681
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 89
  • 1000
    COMPATIBLE WITH RFC[1000]


[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007