Difference between revisions of "Part:BBa K1976055"
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        <h1>trypsin fragment of Colicin E2 with B0034 and T7 promoter</h1>
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Generator for the Colicin DNase domain <a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a>.
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<img style="width: 40%; height: 40%; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/2016/e/ed/T--TU_Darmstadt--minicolgrowth.png">
  
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<partinfo>BBa_K1976055 short</partinfo>
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<b>Figure 1:</b>Figure 5: growth of BL21 E. coli transformed with a): BBa_K1976054 and b): BBa_K1976055.
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T7 generator of the trypsin fragment of the Colicin E2 DNase
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===Usage and Biology===
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===<h2>Usage</h2>===
  
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1976055 SequenceAndFeatures</partinfo>
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This part exhibited signs of leakage, that can kill the host if cells are transformed with it, that have a T7 promoter system, due to the endonuclease activity of the Colicin E2 DNase domain.
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===<h2>Characteristics</h2>===
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===Functional Parameters===
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<partinfo>BBa_K1976055 parameters</partinfo>
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<h2>Sequence and Features</h2>
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<partinfo>BBA_K1976055 SequenceAndFeatures</partinfo>
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<b>References</b>
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[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br>
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Revision as of 15:09, 25 October 2016

trypsin fragment of Colicin E2 with B0034 and T7 promoter

Generator for the Colicin DNase domain BBa_K1976048.

Figure 1:Figure 5: growth of BL21 E. coli transformed with a): BBa_K1976054 and b): BBa_K1976055.


Usage

This part exhibited signs of leakage, that can kill the host if cells are transformed with it, that have a T7 promoter system, due to the endonuclease activity of the Colicin E2 DNase domain.

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 84
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007