Difference between revisions of "Part:BBa K2070012"
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It contains the same part as <partinfo>BBa_K847211</partinfo>, however, instead of in the RFC[25] it is designed in RFC[10]. | It contains the same part as <partinfo>BBa_K847211</partinfo>, however, instead of in the RFC[25] it is designed in RFC[10]. | ||
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===Usage and Biology=== | ===Usage and Biology=== | ||
Reconstructed in the RFC[10] format, the Pnrd promoter was ligated in front of part BBa_E0240 and compared to the standard promoter BBa_J23101. The measurement was made approximately 4h after 100 fold dilution in M9 in a 5 min interval. For details of our characterisation, refer to our protocol. | Reconstructed in the RFC[10] format, the Pnrd promoter was ligated in front of part BBa_E0240 and compared to the standard promoter BBa_J23101. The measurement was made approximately 4h after 100 fold dilution in M9 in a 5 min interval. For details of our characterisation, refer to our protocol. | ||
[[File:T--UT-Tokyo--K2070012.png|thumb|centre|700px|'''Measurement of nrd Promoter Activity.'''The fluorescence from the Pnrd promoter is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.]] | [[File:T--UT-Tokyo--K2070012.png|thumb|centre|700px|'''Measurement of nrd Promoter Activity.'''The fluorescence from the Pnrd promoter is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.]] | ||
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<span class='h3bb'>Sequence and Features</span> | <span class='h3bb'>Sequence and Features</span> | ||
<partinfo>BBa_K2070012 SequenceAndFeatures</partinfo> | <partinfo>BBa_K2070012 SequenceAndFeatures</partinfo> | ||
Latest revision as of 07:01, 25 October 2016
Pnrd promoter
This promoter is activated during cell division. It contains the same part as BBa_K847211, however, instead of in the RFC[25] it is designed in RFC[10].
Usage and Biology
Reconstructed in the RFC[10] format, the Pnrd promoter was ligated in front of part BBa_E0240 and compared to the standard promoter BBa_J23101. The measurement was made approximately 4h after 100 fold dilution in M9 in a 5 min interval. For details of our characterisation, refer to our protocol.
Measurement of nrd Promoter Activity.The fluorescence from the Pnrd promoter is compared with that from the standard promoter BBa_J23101, 3 samples were measured every 9 minutes for each construct. Although there seems to be a slight sudden rise in the promoter activity from the 10-20th minute after the start of measurement, since the change in cell concentration could not be measured simultaneously with fluorescence, it is not certain whether the rise is influenced by the cell cycle/division.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]