Difference between revisions of "Part:BBa K2043001:Design"
Line 8: Line 8:
 
===Design Notes===
 
===Design Notes===
 
His-tag is added in the C-terminal for optimised protein purification
 
His-tag is added in the C-terminal for optimised protein purification
−
<i>catA</i> was codon optimised for <i>E. coli</i>.
+
<i>catA</i> was codon optimised for <i>E. coli</i> and restriction sites for BsaI were avoided.
  
 
===Source===
 
===Source===
  
−
<i>Acinetobacter pittii</i>'s genome
+
<i>Acinetobacter pittii</i>'s genome,
 +
NCBI Reference Sequence for the gene: YP_004995593.1
  
 
===References===
 
===References===
 
Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433. <br><br>
 
Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433. <br><br>
−
 
−
NCBI Reference Sequence: YP_004995593.1
 

Revision as of 21:24, 26 October 2016


catA from Acinetobacter pittii, codon optimized for E. coli


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

His-tag is added in the C-terminal for optimised protein purification catA was codon optimised for E. coli and restriction sites for BsaI were avoided.

Source

Acinetobacter pittii's genome, NCBI Reference Sequence for the gene: YP_004995593.1

References

Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433.