Difference between revisions of "Part:BBa K2043001:Design"
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His-tag is added in the C-terminal for optimised protein purification | His-tag is added in the C-terminal for optimised protein purification | ||
<i>catA</i> was codon optimised for <i>E. coli</i>. | <i>catA</i> was codon optimised for <i>E. coli</i>. | ||
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===Source=== | ===Source=== | ||
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===References=== | ===References=== | ||
+ | Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433. <br><br> | ||
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+ | NCBI Reference Sequence: YP_004995593.1 |
Revision as of 11:33, 24 October 2016
catA from Acinetobacter pittii, codon optimized for E. coli
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
His-tag is added in the C-terminal for optimised protein purification catA was codon optimised for E. coli.
Source
Acinetobacter pittii's genome
References
Lin, J., & Milase, R. N. (2015). Purification and Characterization of Catechol 1, 2-Dioxygenase from Acinetobacter sp. Y64 Strain and Escherichia coli Transformants. The protein journal, 34(6), 421-433.
NCBI Reference Sequence: YP_004995593.1