Difference between revisions of "Part:BBa K2027001"
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__NOTOC__ | __NOTOC__ | ||
<partinfo>BBa_K2027001 short</partinfo> | <partinfo>BBa_K2027001 short</partinfo> | ||
+ | |||
+ | This part was created using a synthetically produced chromoprotein from the DNA2.0 chromoprotein paintbox [1] and adding a cellulose binding domain from the iGEM registry (<partinfo>BBa_K1321366</partinfo>) and a FLAG, lumino and 6x histidine tag. | ||
+ | |||
Learn more about our chromoprotein collection here: http://2016.igem.org/Team:Stanford-Brown/SB16_BioSensor_Chromoproteins. | Learn more about our chromoprotein collection here: http://2016.igem.org/Team:Stanford-Brown/SB16_BioSensor_Chromoproteins. | ||
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<partinfo>BBa_K2027001 parameters</partinfo> | <partinfo>BBa_K2027001 parameters</partinfo> | ||
<!-- --> | <!-- --> | ||
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+ | ==References== | ||
+ | [1] https://www.dna20.com/products/protein-paintbox#3 |
Revision as of 09:07, 25 October 2016
AE Blue-CBD-FlagLumioHisTag Fusion Protein
This part was created using a synthetically produced chromoprotein from the DNA2.0 chromoprotein paintbox [1] and adding a cellulose binding domain from the iGEM registry (BBa_K1321366) and a FLAG, lumino and 6x histidine tag.
Learn more about our chromoprotein collection here: http://2016.igem.org/Team:Stanford-Brown/SB16_BioSensor_Chromoproteins.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 740