Difference between revisions of "Part:BBa K2027004"
Line 17: Line 17:
 
<partinfo>BBa_K2027004 parameters</partinfo>
 
<partinfo>BBa_K2027004 parameters</partinfo>
 
<!-- -->
 
<!-- -->
 +
 +
===Characterization===
 +
 +
Below is the forward and reverse sequence verification of the construct, showing the elastin cross-linking domain, respective heterotrimeric coiled-coil, and bacterial collagen domain.
 +
 +
https://static.igem.org/mediawiki/parts/6/6d/T--Stanford-Brown--c1seqver.png

Revision as of 10:12, 24 October 2016


Bacterial Collagen with Elastin Cross-linker and Coiled-Coil Heterotrimerization Domain 1

This part depends on parts BBa_K2027005 and BBa_K2027006 to function properly. See the design page or the relevant wiki[http://2016.igem.org/Team:Stanford-Brown/SB16_BioMembrane_Collagen] for more details.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 874
    Illegal BsaI.rc site found at 785
    Illegal SapI site found at 175
    Illegal SapI.rc site found at 70


Characterization

Below is the forward and reverse sequence verification of the construct, showing the elastin cross-linking domain, respective heterotrimeric coiled-coil, and bacterial collagen domain.

T--Stanford-Brown--c1seqver.png