Difference between revisions of "Part:BBa K1976048"
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        <h1>Colicin E2 immunity protein</h1>
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This part encodes for the wild-type immunity protein inhibiting the nicking DNase Colicin E2. According to molecular dynamics simulations this protein inhibits also miniColicin (<a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a> and <a href="https://parts.igem.org/Part:BBa_K1976049">BBa_K1976049</a>).
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<img style="width: 40%; height: 40%; margin-left: 15px; margin-right: 15px;" alt="" src="https://static.igem.org/mediawiki/parts/9/9b/Minicolicin.png">
  
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<partinfo>BBa_K1976048 short</partinfo>
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<b>Figure 1:</b>Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.
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The DNase domain of Colicin E2, without both of its other domains.
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===<h2>Usage</h2>===
  
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===Usage and Biology===
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Colicin E2 is a peptide, toxic to coliform bacteria. It is one of a group of proteins of this type, it being a nicking endonuclease. It has a corresponding activity inhibiting protein called immunity protein Im2, which is also in the registry under the name BBa_K1976027.
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The Colicin E2 immunity protein can be used in combination with the Colicin E2 DNase or its engineered truncated variant miniColicin (<a href="https://parts.igem.org/Part:BBa_K1976048">BBa_K1976048</a> and <a href="https://parts.igem.org/Part:BBa_K1976049">BBa_K1976049</a>).
  
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===<h2>Characteristics</h2>===
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K1976048 SequenceAndFeatures</partinfo>
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<h2>Sequence and Features</h2>
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===Functional Parameters===
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<partinfo>BBa_K1976048 parameters</partinfo>
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<b>References</b>
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[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007<br>
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Revision as of 16:35, 23 October 2016

Colicin E2 immunity protein

This part encodes for the wild-type immunity protein inhibiting the nicking DNase Colicin E2. According to molecular dynamics simulations this protein inhibits also miniColicin (BBa_K1976048 and BBa_K1976049).

Figure 1:Structure of Colicin E2 DNase domain derived from a molecular dynamics simulation.


Usage

The Colicin E2 immunity protein can be used in combination with the Colicin E2 DNase or its engineered truncated variant miniColicin (BBa_K1976048 and BBa_K1976049).

Characteristics

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 150
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References
[1] Cascales et al, Colicin Biology, Microbiology and Molecular Biology Reviews, vol. 71, pp. 158-229, 2007