Difference between revisions of "Part:BBa K1893018"
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<partinfo>BBa_K1893018 short</partinfo> | <partinfo>BBa_K1893018 short</partinfo> | ||
| − | + | The leucine biosynthesis gene placed under control of a pBAD promoter, used to control growth of an E. coli leucine auxotroph using arabinose. | |
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===Usage and Biology=== | ===Usage and Biology=== | ||
Revision as of 06:56, 26 October 2016
Arabinose inducible 3-isopropylmalate dehydrogenase (pBAD+leuB)
The leucine biosynthesis gene placed under control of a pBAD promoter, used to control growth of an E. coli leucine auxotroph using arabinose.
Usage and Biology
The leucine biosynthesis pathway is activated in conditions of leucine starvation and can be manipulated for the purposes of auxotrophy experiments. We initially considered auxotrophy as a method of controlling growth through our growth-regulation module. This part was designed to investigate the effect of leuB suppression on the growth of E. coli leuB knockout strains. The knockout strains were to be grown in minimal media lacking leucine, so that only cells expressing leuB at a sufficient level would be capable of growth. Due to time constraints, and the difficulty of creating knockout strains, we were unable to obtain any characterisation data.
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1342
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 1281
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1116
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI site found at 1098