Difference between revisions of "Part:BBa K1963010:Design"

Latest revision as of 13:21, 23 October 2016

An sRNA system for down-regulation of E. coli fliC encoding flagellin

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI.rc site found at 101

Design Notes

This part is RFC[10] compliant

Source

This part was generated by PCR using phosphorylated primers and BBa_K1963002 as template.

References

Erhardt M. (2016) Strategies to Block Bacterial Pathogenesis by Interference with Motility and Chemotaxis. Curr Top Microbiol Immunol [Epub ahead of print] doi:10.1007/82_2016_493

De Maayer P, Cowan DA. (2016) Flashy flagella: flagellin modification is relatively common and highly versatile among the Enterobacteriaceae. BMC Genomics 17:377. doi: 10.1186/s12864-016-2735-x.

Pushkin DO, Bees MA. (2016) Bugs on a Slippery Plane : Understanding the Motility of Microbial Pathogens with Mathematical Modelling. Adv Exp Med Biol 915:193-205. doi: 10.1007/978-3-319-32189-9_12.

Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols 8, 1694–1707. doi:10.1038/nprot.2013.105

@@ Line 21: / Line 21: @@
 Pushkin DO, Bees MA. (2016) Bugs on a Slippery Plane : Understanding the Motility of Microbial Pathogens with Mathematical Modelling. <b><i>Adv Exp Med Biol</i> 915:</b>193-205. doi: 10.1007/978-3-319-32189-9_12.
+Seung Min Yoo, Dokyun Na & Sang Yup Lee (2013) Design and use of synthetic regulatory small RNAs to control gene expression in <i>Escherichia coli</i>. <b><i>Nature Protocols</i> 8,</b> 1694–1707.
+doi:10.1038/nprot.2013.105