Difference between revisions of "Part:BBa K1893010"
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<partinfo>BBa_K1893010 short</partinfo> | <partinfo>BBa_K1893010 short</partinfo> | ||
| − | This is the STAR construct under control of the constitutive promoter j23119. When transcribed, it produces the STAR-antisense RNA (pAD1.A5) to the sense target attenuator sequence (pAD1.S5). | + | This is the STAR construct under control of the constitutive promoter j23119. When transcribed, it produces the STAR-antisense RNA (pAD1.A5), which can bind to the sense STAR-target attenuator sequence (pAD1.S5). Thus, only in the presence of STAR-antisense, transcription and expression of coding sequences downstream to the STAR-target is activated |
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Revision as of 11:54, 23 October 2016
Constitutive STAR (J23119+STAR)
This is the STAR construct under control of the constitutive promoter j23119. When transcribed, it produces the STAR-antisense RNA (pAD1.A5), which can bind to the sense STAR-target attenuator sequence (pAD1.S5). Thus, only in the presence of STAR-antisense, transcription and expression of coding sequences downstream to the STAR-target is activated
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]