Difference between revisions of "Part:BBa K1963007"

Revision as of 09:52, 23 October 2016

Device for rhamnose-dependent expression of an OsmY.

An assembled device that contains a rhamnose-responsive promoter, a strong RBS and a biobrick encoding the E. coli OsmY protein.

Usage and Biology

The E. coli OsmY protein is really interesting. Originally dubbed "osmotically-induced protein Y" it is synthesised with an N-terminal signal peptide (MTMTRLKISKTLLAVMLTSAVATGSAYA) most likely of the Sec type. While the protein has been reported to be periplasmic, it also appears to be one of the few fully secreted proteins that E. coli K-12 synthesises. This property has been used in SynBio projects (e.g. Steen et al. 2010) and in protein production projects (e.g. Le & Wang 2014).

An OsmY-encoding biobrick was assembled by Washington iGEM in 2012 (

Figure 1: Whole cell (A) and supernatant blots (B) of prhamnose-OsmY-HA (C) in the presence of increasing concentrations of Rhamnose. Anti-HA immunblot shows whole cell expression and secretion of OsmY-HA in the presence of Rhamnose.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 7: / Line 7: @@
 ===Usage and Biology===
-The <i>E. coli</i> OsmY protein is really interesting.
+The <i>E. coli</i> OsmY protein is really interesting. Originally dubbed "osmotically-induced protein Y" it is synthesised with an N-terminal signal peptide (MTMTRLKISKTLLAVMLTSAVATGSAYA) most likely of the Sec type. While the protein has been reported to be periplasmic, it also appears to be one of the few fully secreted proteins that <i>E. coli</i> K-12 synthesises. This property has been used in SynBio projects (e.g. Steen <i>et al.</i> 2010) and in protein production projects (e.g. Le & Wang 2014).
+An OsmY-encoding biobrick was assembled by Washington iGEM in 2012 (