Difference between revisions of "Part:BBa K1963009"
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Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no lysis.  
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Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.  
  
  

Revision as of 22:29, 22 October 2016


Device for rhamnose-dependent expression of an OsmY fusion to Serratia Hfq.

A rhamnose-regulated device based on existing and new (2016) biobricks. This is designed to induce production of a fusion protein between E. coli OsmY (a secerted protein) and Serratia marcescens Hfq (an RNA binding protein). Co-expression with Serratia sRNA may generate a specific RNA secretion system.


Usage and Biology

Hfq-serratia-frulhuq4.png

Figure 1. Whole cell blots expressing OsmY-Hfq fusion protein under the control of the rhamnose promoter.

Allsupernatant_blots2.png

Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]