Difference between revisions of "Part:BBa K1963008"
Line 10: Line 10:
  
 
https://static.igem.org/mediawiki/parts/6/67/Hfq-serratia-frulhuq5.png
 
https://static.igem.org/mediawiki/parts/6/67/Hfq-serratia-frulhuq5.png
 +
 +
Figure 1. Whole cell blots expressing OsmY-Hfq (E.coli) fusion protein under the control of the rhamnose promoter.
 +
 +
 +
https://static.igem.org/mediawiki/parts/8/81/Allsupernatant_blots2.png
 +
 +
Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.
  
  

Revision as of 22:31, 22 October 2016


Device for rhamnose-dependent expression of an OsmY fusion to E. coli Hfq..

A composite of three biobricks to give rhamnose-regulated expression of an sRNA secretion system - a translational fusion between E. coli OsmY and E. coli Hfq.


Usage and Biology


Hfq-serratia-frulhuq5.png

Figure 1. Whole cell blots expressing OsmY-Hfq (E.coli) fusion protein under the control of the rhamnose promoter.


Allsupernatant_blots2.png

Figure 2. Supernatant blots of OsmY-HA, OsmY-Hfq (E. coli) and OsmY-Hfq (Serratia). Western blotting with anti-HA antibody shows secretion of OsmY-HA, and OsmY-Hfq (E. coli) and anti-RNAP antibody shows no antigen detected in the supernatant therefore the cells remained intact and there was no cell lysis.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]