Difference between revisions of "Part:BBa K1893002:Experience"

Latest revision as of 13:54, 27 October 2016

Applications of BBa_K1893002

We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.

This part worked as expected, and when we placed GFP downstream of the RhlR promoter, we saw fluorescence when we induced with C4 AHL.

User Reviews

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-We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.
-This part worked as expected, and when we placed GFP downstream of the RhlR promoter, we saw fluorescence when we induced with C4 AHL.
 ===Applications of BBa_K1893002===
+We used this part to engineer a bacterial communication system which was bidirectional, so that two different strains could grow together. Each strain in our system must have a distinct HSL associated with it, so we required two quorum sensing systems which were orthogonal. We found that Las and Rhl had very little crosstalk, indicating that they were good candidates for our system.
+This part worked as expected, and when we placed GFP downstream of the RhlR promoter, we saw fluorescence when we induced with C4 AHL.
 ===User Reviews===
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