Difference between revisions of "Part:BBa K1962003"

Revision as of 19:11, 22 October 2016

Colicin Ia::Ssp2 Chimera

This part contains an translational fusion protein between the truncated bacteriocin-free version of Colicin Ia (BBa_K1962002) and an antibacterial toxin (peptidoglycan hydrolase) called Ssp2 from Serratia marcescens. Ssp2 is an antibacterial effector normally secreted by the Type VI secretion system (T6SS) in Serratia marcescens and it has peptidoglycan endopeptidase activity, degrading peptidoglycan in the periplasm of the target cell where it cleaves between y-D-glutamic acid and L-mesodiaminopimelic acid in peptidoglycan.

Usage and Biology

File:Https://static.igem.org/mediawiki/2016/2/22/T--Dundee--Results23.png

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
INCOMPATIBLE WITH RFC[12]
Illegal NheI site found at 1858
21
INCOMPATIBLE WITH RFC[21]
Illegal BamHI site found at 1351
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

Revision as of 19:11, 22 October 2016 (view source) Frulhuq (Talk \| contribs) ← Older edit		Revision as of 19:11, 22 October 2016 (view source) Frulhuq (Talk \| contribs) Newer edit →
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