Difference between revisions of "Part:BBa K1893001:Design"
 
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
This part does not include an LVA tag on the LasR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pLas promoter, activating transcription of downstream genes. This part contains everything necessary for AHL-induced expression of genes inserted downstream of the pLas promoter. GFPmut3b was included in this part so that we could characterize the activation range of the LasR system.  
+
This part does not include an LVA tag on the LasR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pLas promoter, activating transcription of downstream genes. This part contains everything necessary for 3O-C12-HSL-induced expression of genes inserted downstream of the pLas promoter, which is, in this case, GFPmut3b. GFPmut3b was included in this part so that we could characterize the activation range of the LasR system.  
  
  

Latest revision as of 19:18, 22 October 2016


Las receiver with GFP reporter (LasR+pLas+GFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 383
    Illegal AgeI site found at 580
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1719


Design Notes

This part does not include an LVA tag on the LasR gene. This means that the protein is not rapidly degraded. When active, the LasR protein binds to the pLas promoter, activating transcription of downstream genes. This part contains everything necessary for 3O-C12-HSL-induced expression of genes inserted downstream of the pLas promoter, which is, in this case, GFPmut3b. GFPmut3b was included in this part so that we could characterize the activation range of the LasR system.


Source

Freemont Lab, Imperial College London

References