Difference between revisions of "Part:BBa K2074026"
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our part is coding the EGFPE,We transferred the gene EGFP into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein and to test whether our systerm work or not.
 
our part is coding the EGFPE,We transferred the gene EGFP into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein and to test whether our systerm work or not.
  
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[[File:T--FAFU-CHINA--Result4.png | 825px | thumb | center ]]
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[[File:T--FAFU-CHINA--Result4.png | 825px | thumb | left ]]
 
https://static.igem.org/mediawiki/2016/thumb/0/05/T--FAFU-CHINA--Result4.png/800px-T--FAFU-CHINA--Result4.png
 
https://static.igem.org/mediawiki/2016/thumb/0/05/T--FAFU-CHINA--Result4.png/800px-T--FAFU-CHINA--Result4.png
 
https://static.igem.org/mediawiki/2016/thumb/7/72/T--FAFU-CHINA--Result5.png/800px-T--FAFU-CHINA--Result5.png
 
https://static.igem.org/mediawiki/2016/thumb/7/72/T--FAFU-CHINA--Result5.png/800px-T--FAFU-CHINA--Result5.png

Revision as of 04:04, 22 October 2016


EGFP(Codon optimization)

our part is coding the EGFPE,We transferred the gene EGFP into plasmid ,and transformed the recombinant plasmid into Chlamydomonas reinhardti to express protein and to test whether our systerm work or not.

T--FAFU-CHINA--Result4.png

800px-T--FAFU-CHINA--Result4.png 800px-T--FAFU-CHINA--Result5.png 800px-T--FAFU-CHINA--Result6.png


T--FAFU-CHINA--EGFPoptimization.png

T--FAFU-CHINA--EGFPoptimization2.png


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 682
  • 1000
    COMPATIBLE WITH RFC[1000]
File:T--FAFU-CHINA--E.png
Figure.1 The gel electrophoresis result of EGFP