Difference between revisions of "Part:BBa K1943025:Design"
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Latest revision as of 03:31, 22 October 2016
TRPC5-18
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 930
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 167
Illegal BglII site found at 1520 - 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 2539
Illegal BsaI.rc site found at 1627
Illegal BsaI.rc site found at 2054
Illegal SapI site found at 1722
Design Notes
This year, TRPC5 takes a quite important role in our project(see how we do the directed evolution experiment: http://2016.igem.org/Team:SUSTech_Shenzhen/Notebook/Molecular ). Then we design primers and do PCR of this TRPC5 coding sequence and ligate it to pSB1C3 backbone.
Source
Original TRPC5：
Sequence from NCBI
Synthesis by IDT and Wuxi Qinglan Biotech Co. Ltd.